Western Blot:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Immunohistochemistry:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Ubiquitin Proteomics:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Immunopeptidomics:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Flow Cytometry:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Immunofluorescence:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Immunoprecipitation:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
Membrane:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
RNA Immunoprecipitation:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
RNA Binding Assay:Article Title: m 6 A‐modified EHD1 controls PD‐L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma
Article Snippet: Name Catalog No. Company Application Working dilution EHD1 A02168-2 Boster WB 1:1,000 24657-1-AP Proteintech WB 1:1,000 24657-1-AP Proteintech IHC 1:200 Bax 50599-2-Ig Proteintech WB 1:2,000 Bcl-2 26593-1-AP Proteintech WB 1:2,000 PD-L1 66248-1-Ig Proteintech WB 1:5,000 66248-1-Ig Proteintech IHC 1:3,000 66248-1-Ig Proteintech IF 1:300 PE-PD-L1 PE-65073 Proteintech FCM 1:40 YTHDF1 66745-1-Ig Proteintech WB 1:2,000 66745-1-Ig Proteintech IHC 1:2,000 66745-1-Ig Proteintech RIP 1:320 LAMP1 21997-1-AP Proteintech IF 1:100 RAB11 15903-1-AP Proteintech IF 1:100 IgG 66360-1-Ig Proteintech FCM 1:200 66360-1-Ig Proteintech IP 1:200 CD8 66868-1-Ig Proteintech IHC 1:5,000 Ubiquitin 10201-2-AP Proteintech WB 1:1,000 Flag-Tag 20543-1-AP Proteintech IP, WB 1:2,000 HA-Tag 66006-2-Ig Proteintech WB 1:2,000 Myc-Tag 16286-1-AP Proteintech IP, WB 1:2,000 β-actin TA-09 Zhongshan Jinqiao WB 1:1,000 MHC-I 17260-1-AP Proteintech WB 1:2,000 FGL1 16000-1-AP Proteintech WB 1:800 CD155 66913-1-Ig Proteintech WB 1:2,000 PARP 66520-1-Ig Proteintech WB 1:40,000 Caspase 3 #9662S Cell Signaling WB 1:1,000 Cleaved-Caspase 3 ab32042 abcam WB 1:500 GAPDH 60004-1-Ig Proteintech WB 1:4,000 HRP-conjugated goat anti-rabbit secondary antibodies ZB2301 Zhongshan Jinqiao WB 1:10,000 HRP-conjugated goat ZB-2305 Zhongshan Jinqiao WB 1:10,000 anti-mouse secondary antibodies CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) SA00013-4 Proteintech IF 1:100 Fluorescein (FITC)-conjugated Affinipure Goat Anti-Mouse IgG (H+L) SA00003-1 Proteintech IF 1:50 Abbreviations: Bax, Bcl-2 associated X; Bcl-2, B-cell lymphoma-2; CD8, Cluster of differentiation 8; CD155, Cluster of differentiation 155;EHD1, Eps15 homology domain 1; FCM, Flow cytometry; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; HRP, Horseradish Peroxidase; IF, Immunofluorescence; IHC, Immunohistochemistry; IP, Immunoprecipitation; LAMP1, Lysosome-associated membrane proteins-1; MHC-I, Major histocompatibility complex I; PARP, Poly ADP-ribose polymerase; PD-L1, Programmed cell death 1 ligand 1; RAB11, Ras-related protein rab11a-like protein; RIP, RNA immunoprecipitation; WB, Western blotting; YTHDF1, YTH N6-methyladenosine RNA binding protein F1.
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